[MassChestnutOrchards] Recent Roundup research

[Yvonne Federowicz]

Endocrine disruption is not generally fatal to the individual exposed, so
the LD50 test is inappropriate. It¹s more a problem with reproduction.

This is the study ­ it mentions ³cytotoxicity² and ³endocrine disruption².
While I¹m not an endocrinologist or toxologist, I am glad that foreign
countries & the EPA are going to study these chemicals more ­ there are a
lot of negative things happening to wildlife (and human) endocrine systems
right now that no one can explain adequately, and relying upon
company-sponsored studies strikes me as highly dubious.

A number of plastic-related compounds took years to be banned for endocrine
disruption but finally were, so at the least, caution is indicated.

------------------

Glyphosate-based herbicides are toxic and endocrine disruptors in human cell
lines 

 Céline Gasniera,  Coralie Dumontb  , Nora Benachoura , Emilie Claira  ,
Marie-Christine Chagnonb  and Gilles-Eric Séralinia
aUniversity of Caen, Institute of Biology, Lab. Biochemistry EA2608,
Esplanade de la Paix, 14032 Caen cedex, France

bUniversity of Burgundy, Lab. Food Toxicology UMR1129, 1 Esplanade Erasme,
21000 Dijon, France
 

Received 10 April 2009; 
revised 4 June 2009; 
accepted 8 June 2009. 
Available online 17 June 2009.

Abstract
Glyphosate-based herbicides are the most widely used across the world; they
are commercialized in different formulations. Their residues are frequent
pollutants in the environment. In addition, these herbicides are spread on
most eaten transgenic plants, modified to tolerate high levels of these
compounds in their cells. Up to 400 ppm of their residues are accepted in
some feed. We exposed human liver HepG2 cells, a well-known model to study
xenobiotic toxicity, to four different formulations and to glyphosate, which
is usually tested alone in chronic in vivo regulatory studies. We measured
cytotoxicity with three assays (Alamar Blue®, MTT, ToxiLight®), plus
genotoxicity (comet assay), anti-estrogenic (on ER?, ER?) and
anti-androgenic effects (on AR) using gene reporter tests. We also checked
androgen to estrogen conversion by aromatase activity and mRNA. All
parameters were disrupted at sub-agricultural doses with all formulations
within 24 h. These effects were more dependent on the formulation than on
the glyphosate concentration. First, we observed a human cell endocrine
disruption from 0.5 ppm on the androgen receptor in MDA-MB453-kb2 cells for
the most active formulation (R400), then from 2 ppm the transcriptional
activities on both estrogen receptors were also inhibited on HepG2.
Aromatase transcription and activity were disrupted from 10 ppm. Cytotoxic
effects started at 10 ppm with Alamar Blue assay (the most sensitive), and
DNA damages at 5 ppm. A real cell impact of glyphosate-based herbicides
residues in food, feed or in the environment has thus to be considered, and
their classifications as carcinogens/mutagens/reprotoxics is discussed.


On 1/8/10 1:55 PM, "Guy Shepard" <tsuga35 at aol.com> wrote:

> The LD50  is about 27000 compare that to asprin .
> Guy 
> 
> 
> 
> -----Original Message-----
> From: Yvonne Federowicz <Yvonne_Federowicz at brown.edu>
> To: masschestnutorchards at masschestnut.org
> Sent: Fri, Jan 8, 2010 1:12 pm
> Subject: Re: [MassChestnutOrchards] Recent Roundup research
> 
> I found blog pages saying that Australia has banned Glyphosphate "near"
> water.  Denmark seems to have banned it completely, and there is discussion
> that the EU and Argentina might ban it.  It's hard to evaluate the truth of
> some of the blog sources I found online though.
> 
> The EPA is apparently starting screening of Glyphosphate for endocrine
> disruption:
> 
> http://www.epa.gov/scipoly/oscpendo/pubs/final_list_frn_041509.pdf
> (discussed at: 
> http://www.democraticunderground.com/discuss/duboard.php?az=view_all&address
> =115x193628)
> 
> 
> On 1/8/10 1:03 PM, "Fred Hebard" <Fred at acf.org> wrote:
> 
>> > This site appears to be selling it at present:
>> > 
>> > http://www.scottsaustralia.com.au/Roundup
>> > 
>> > Fred
>> > 
>> > Frederick V. Hebard, PhD
>> > Staff Pathologist, Meadowview Research Farms
>> > American Chestnut Foundation
>> > 14005 Glenbrook Ave.
>> > Meadowview, VA 24361
>> > 
>> > Email: Fred at acf.org
>> > Web: http://www.acffarms.org <http://www.acffarms.org/>
>> > Phone: (276) 944-4631
>> > Fax: (276) 944-0934
>> > 
>> > 
>> > On Jan 8, 2010, at 12:50 PM, Brian A Pistolese wrote:
>> > 
>>> >> 
>>> >> I'm fairly certain that the use of 'Roundup' has been banned in
>>> >> Australia.
>>> >> _______________________________________________
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>> > 
>> > _______________________________________________
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